Posted by admin on Jan 13, 2018 in |
A simple, rapid, accurate, precise, specific, sensitive and stability indicating RP-HPLC method has been developed and validated for the simultaneous estimation of Metformin and Empagliflozin in bulk drug and pharmaceutical dosage form. The chromatographic separation was performed on the Kromasil C18 column (250mm×4.6mm, 5μm particle size), using a mobile phase of buffer: Acetonitrile taken in the ratio 45:55 v/v, at a flow rate of 1.1 ml/min at an ambient temperature of 30˚C with the detection wavelength at 226nm. The retention times of Metformin and Empagliflozin were 2.182 min and 2.908 min, respectively. The linearity was performed in the concentration range of 125-750 ppm, 3.12-18.75 ppm each of Metformin and Empagliflozin with a correlation coefficient of 0.999 and 0.999 for Metformin and Empagliflozin respectively. The percentage purity of Metformin and Empagliflozin was found to be 100.59% and 100.07% respectively. The proposed method has been validated for specificity, linearity, range, accuracy, precision, and robustness were within the acceptance limit according to ICH Q2 (R1) guidelines, and the developed method can be...
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Posted by admin on Nov 28, 2016 in |
Aim and objective: The aim of the in-vitro study is to evaluate the Antimicrobial activity of root canal sealant- antibiotic combination on Enterococcus feacalis. Background: Elimination of microorganisms from the root canal system is the one of the most important objectives of RCT. Enterococcus faecalis (EF) has been the most frequently identified species in canals of root-filled teeth with periapical lesions. EF is extremely resistant to current treatment modalities in endodontics. Therefore, the success rate of root canal treatment can be significantly reduced with the presence of this microorganism at the time of obturation. So the root canal sealant along with antibiotic combinations are used to inhibit and kill the bacteria present in the surface walls of the canals. Methodology: The antimicrobial activity of root canal sealer- antibiotic combination on Enterococcus faecalis was tested agar well diffusion method. Results: Both the sealers tested in combination with clindamycin and amoxicillin showed greater zone of inhibition when compared with sealer with single antibiotic...
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Posted by admin on Nov 28, 2016 in |
Type 2 diabetes mellitus (T2DM) consider the most common diseases in modern societies. Natural products or compounds reported as useful remedies for controlling or preventing T2DM are categorized into 5 major groups namely, anti-inflammation products, AMPK activators, insulin secretion stimulators, alpha-glucosidase/ disaccharidase or amylase inhibitors and products acting with an unknown mechanism. Evening primrose oil is a substantial source of omega-6 essential fatty acids, mostly gamma-linolenic acid (GLA)). Linolenic acid (LA) forms GLA by Δ-6-desaturase enzyme. The activity of Δ-6-desaturase enzyme, which is compromised in patients with type 2 diabetes. Accordingly, this study aims to evaluate the effect of evening primrose oil in reducing the complications of type 2 diabetes mellitus. Twenty-Six Iraqi patients newly diagnosed with type 2 diabetes who are either overweight or obese. Thirteen patients received metformin 500 mg tablets twice daily alone, and 13 patients received metformin 500 mg plus evening primrose oil 2 gm capsule twice daily for 3 month therapy. Serum hs-CRP, Tumor necrosis factor α, and malondialdehyde (MDA) were measured. There was...
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Posted by admin on Dec 13, 2015 in |
Background: Recently, in the discipline of ophthalmology, there have been many developments and introduction of new ocular therapeutic agents. To improve drugs therapeutic efficacy, minimize adverse effects, and delay development of resistance drug utilization trends and patients need to be evaluated periodically. Objective: To assess the prescribing, drug use, and to dispense practice of ophthalmic drugs at Boru hospital. Methodology: Cross-sectional study was conducted on patients attending outpatient department pharmacy of Boru Meda hospital to collect their medicines. Prescriptions of 84 patients encountered were analyzed using World Health Organization (WHO) prescribing indicators and additional indices. Result: Analysis showed that the mean number of drug per prescription was 2.2 and 97.2% of prescribed drugs were from the national essential drug list. 89.5% of drugs were prescribed by their generic name. Majority of the prescribed drugs were antibiotics (62.7%), and only 17.7% of the prescriptions had a frequency of dosing. Percentage of patients with good post-dispensing knowledge on the dispensed ophthalmic drugs was 37.1%. Dispensing and counseling time were 18sec and...
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Posted by admin on Dec 13, 2015 in |
This prescription survey was carried out to find the most frequently used benzodiazepine class (e.g., Clonazepam, Diazepam, Bromazepam, Clobazam, Midazolam, Flurazepam & Alprazolam) & their rational use as anti-anxiety agents in the Comilla & Feni District of Bangladesh. All the prescriptions were collected in June 2015, prescribed by psychiatrists throughout May 2015. We have found prescription containing 21% Clonazepam, 12% Flurazepam, 6% Midazolam, 4% Alprazolam, 2% Bromazepam, 2% Diazepam & 2% Clobazam in Comilla district. 51% prescription did not contain any benzodiazepines. In Feni district, we have found 22% Clonazepam, 0% Flurazepam, 0% Midazolam, 11% Alprazolam, 6% Bromazepam, 0% Diazepam & 0% Clobazam in the prescription. 61% prescription in this district did not contain benzodiazepines. We found 1 prescription containing Midazolam & Flurazepam (both are benzodiazepines). In another prescription collected from Feni district, we got 1 prescription containing Clonazepam & Alprazolam. But we recommend the citation of the chief complain or diagnosis in the prescription for better understanding and justification of using any drug, especially when it is a...
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