ANTIMICROBIAL ACTIVITY OF A SELECTED MEDICINAL PLANT EXTRACTS MIXTURE USED IN THE MANAGEMENT OF CHRONIC SKIN WOUNDS IN AYURVEDIC MEDICINEAbstract
The aim of the study was to determine the synergistic antimicrobial activity of a selected medicinal plant extract mixture composed of Calotropis gigantean (ElaWara, Family: Asclepiadaceae), Datura metel (Kalu Attana, Family: Solanaceae) and Ricinus communis (Erandu, Family: Euphorbiaceae) against selected gram positive and gram negative bacteria. In the study, antimicrobial activity was evaluated against two-gram positive organisms; Staphylococcus aureus (ATCC 95923) and group A Streptococcus spp. (ATCC 12384) and two gram-negative organisms; Escherichia coli (ATCC 95922) and Pseudomonas aeruginosa (ATCC 27853). Agar disc diffusion method was carried out to evaluate the antibacterial activity of the selected medicinal plant extract mixture, and the inhibition zone diameters were measured. Further, minimum inhibitory concentration (MIC) was evaluated using a broth microdilution method. The inhibition zones against S. aureus and group A Streptococcus spp. were observed. In contrast, inhibition zones were not demonstrated against selected gram-negative organisms. The MIC evaluated for S. aureus was 16 mg/mL by the broth dilution method. The results revealed that the selected medicinal plant extract mixture exerted synergistic antimicrobial activity against S. aureus and group A Streptococcus spp. However, antimicrobial activity against E. coli and P. Aeruginosa was not significant. Results were able to scrutinize the therapeutic benefits of the selected medicinal plant extracts mixture in the management of chronic skin wounds in Ayurvedic medicine. Also, the plant extract mixture may offer valuable natural drug leads for the development of a commercially viable herbal antimicrobial formulation in the future.
W. G. G. Piumali, A. P. Attanayake * and H. Peiris
Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Karapitiya, Sri Lanka.
16 January 2018
04 February 2018
24 February 2018
28 February 2018