COMPARITIVE ANALYSIS OF PHYTOCHEMICAL COMPOUNDS AND ANTIBACTERIAL ACTIVITY OF CERTAIN UNDERUTILIZED WILD LEGUMESAbstract
The current study was performed to study the antibacterial effects of solvent (ethanol, methanol, chloroform, hexane, acetone, petroleum ether) and aqueous extracts of lima bean, horse gram, and jack bean. The phytochemical studies performed on the extracts revealed the presence of alkaloids, steroids, triterpenoids, flavonoids, tannins, phenols, glycosides, and saponins. The antibacterial activity of these extracts was examined using agar well diffusion method. The extracts were studied for activity against pathogenic bacteria, namely P. syringae, E. coli, B. subtilis, E. faecalis, S. aureus, S. typhi, B. cereus, P. aeruginosa. The activity of the extracts against bacterial proliferation was estimated by measuring the zone of inhibition. The highest antibacterial activity among all the extracts tested and all the seeds involved in this study, the ethanol extract of horse gram has exhibited the maximum antibacterial effect against E. coli (12.0 ± 0.29), E. fecalis (11.0 ± 0.29) and B. substilis (10.0 ± 0.34). The results indicate that all three seeds in the discussion here show high potential for commercialization as antibacterial agents, due to their rich phytochemical contents.
M. Marimuthu *, P. Gurumoorthi, S. Uma and V. Anupama
Nutracuetical Lab, Department of Food Process Engineering, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.
18 February 2016
22 March 2016
29 March 2016
30 April 2016