FORMULATION, OPTIMIZATION AND EVALUATION STUDY FOR AN ALOE EMODIN SYNTHETIC DERIVATIVE 4,5–DIHYDROXY-9,10-DIOXO-9,10-DIHYDROANTHRACENE-2 CARBOXYLIC ACID IN GEL DOSAGE FORMAbstract
Dermatologists should be aware of cosmeceutical products and have access to accurate scientific theory with validated data-if any exists-to support cosmeceutical claims. The question remains whether it is possible to deliver adequate doses to the skin in-vivo and to produce clinical or histological effects. It is important to evaluate these new products with a critical and careful methodology, considering intended product use and the design of available studies supporting product use. The system was optimized with a primary aim to prepare this formulation is to reduce down the concentration of ethanol in formulation by addition of PEG-600 as a co-solvent. All formulations were observed for appearance of color and transparency of gel. The pH of gels was adjusted in the range 6.8 to 7.4. The formulation designed including the ingredients of carbopol 934, PEG (600), ethanol, and water. Evaluation carried out for the formulation with rheological studies. In-vitro diffusion study through flow through diffusion cell method. Drug content uniformity was determined spectrophotometrically. Spreadability indicates that the gel is easily spreadable by small amount of shear. Viscosity (cp) measured by a Brookfield programmable viscometer (RVD-II+) along with helipath assembly. The skin irritation assay was performed following the organization for Economic Cooperation and Development (OECD) Guidelines for Testing of Chemicals, Section 4, No. 404 – Acute Dermal Irritation / Corrosion study, the formulations were found to be non-irritating and free of skin allergy. The gel formulation showed a significant consistency and passes all the relevant stability testing. The formulation further proceeds to pharmacological evaluation study.
A. N. Sharma*, R. Mishra, P. S. Admane and S. M. Mendhi
Department of Pharmacology, Padm. Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, Maharshtra, India
08 May 2015
20 June 2015
27 June 2015
30 June 2015