GROWTH AND NON-ENZYMATIC ANTIOXIDATIVE STUDIES IN IN-VITRO GROWN SAFFLOWER (CV A-1) SEEDLINGS UNDER COPPER STRESSAbstract
In the present study, we were interested in evaluating the effects of copper on morphological features and non- enzymatic antioxidants in the safflower (cv. A-1). The safflower seeds were grown in Hoagland’s solution supplied with the 0.5 µM (control), 25, 50 and 100 µM CuSO4 for 10th and 20th days. The seedlings were harvested after different days (10th and 20th days). The presence of 50 µM and 100 µM concentration of Cu stimulated impaired root growth after 10th and 20th days, while 25 µM Cu was found to be less in inhibiting the root growth. Also, the shoot growth was found to be adversely affected with increased copper concentration. The malondialdehyde content, nonenzymatic antioxidants (flavonoids and polyphenols), and proline were found to be accumulated in both days harvested leaves of safflower’s seedlings. Thus the conclusion reveals that the increase of copper above threshold point affects the plants in a negative manner.
S. Gautam, S. S. Bhagyawant and N. Srivastava *
Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Jaipur, Rajasthan, India.
08 February 2016
10 March 2016
18 March 2016
31 March 2016