OESTROGEN AND THYROXINE MODULATE BLOOD FLOW MECHANISMS IN TADALAFIL-TREATED WISTAR RATSAbstract
We earlier established the anti-secretory and mucogenic activities of tadalafil in indomethacin induced ulceration in rats and that these properties are modulated by the presence of sex hormones and thyroxine. The current study investigated the influence of these hormones on gastric blood flow, prostaglandin secretion and nitric oxide production in rats. Adult Wistar rats (140-160 g) were used. Gastric ulcer was induced in fasted rats using oral indomethacin (40 mg/Kg). Direct gastric blood flow was measured with a transonic T206 blood flow meter. Animals were sacrificed 4 h after ulcer induction. ELISA technique was used to evaluate the concentration of prostaglandin E2 (PGE2) and nitrite in the gastric homogenate. Data were analysed by ANOVA followed by Newman Keul’s post-hoc test at α0.05. Blood flow, gastric PGE2 and nitric oxide contents were significantly elevated by tadalafil and by co-administration of tadalafil with oestrogen or thyroxine. We conclude that the gastro protective effect of tadalafil was mediated by increased PGE2 and nitric oxide generation and that this protective effect is enhanced by the presence of exogenous thyroxine and oestrogen in Wistar rats.
I. A. Kolawole * and S. O. Francis
Department of Physiology, Benjamin S Carson Snr School of Medicine, Babcock University, Ilisan-Remo, Nigeria.
23 October 2019
24 December 2019
27 December 2019
31 December 2019