SIMULTANEOUS ESTIMATION OF IBUPROFEN AND CARISOPRODOL IN SYNTHETIC MIXTURE BY HPTLC METHODAbstract
A Simple, accurate, and precise high-performance thin layer chromatography method for the simultaneous estimation of ibuprofen and carisoprodol in the marketed formulation was developed. The determination was carried on silica gel 60 GF254 HPTLC plates using a mobile phase n-butanol: glacial acetic acid: Acetone (5:2:3 v/v/v). The absorbance of the spots was measured by densitometry at 254 nm. The Retention Factor (Rf) was found to be 0.87 for ibuprofen and 0.77 for carisoprodol. Ibuprofen and carisoprodol showed a linear response in the concentration range 60-180 µg/band and 35-78.75µg/band, respectively. The correlation coefficient (r2) for ibuprofen and carisoprodol was found to be 0.9992 and 0.9997, respectively. The result of the analysis has been validated statistically and by recovery studies. The percentage recoveries obtained for ibuprofen and carisoprodol ranges from 101.92 and 100.46 respectively. The proposed HPTLC method can be applied for the quantitative determination of carisoprodol and ibuprofen in bulk and drug formulation.
C. Patel, L. Prajapati*, A. Joshi and M. Kharodiya
Shri B. M. Shah College of Pharmaceutical Education and Research, Modasa, Gujarat, India.
09 October 2015
20 November 2015
28 November 2015
30 November 2015