Posted by admin on Jun 30, 2018 in |
Most of the existing anticancer drugs are very potent small molecules; their efficacy is constrained by their systemic toxicity, narrow therapeutic window, low drug loading, size control, scale up, cost of formulation but also as a result of drug resistance and limited cellular entry. Due to these obstacles, controlled and targeting or localized release technology has been replacing the systemic administration and has shown lots of potential for cancer treatment. Liposomes can be used to provide a sustained release of drugs, which require a prolonged plasma concentration at therapeutic levels to achieve the optimum therapeutic efficacy. In the present work, we formulate liposome containing the anticancer drug ‘6 Mercaptopurine’ for sustained delivery. The drug incorporation was carried out using the ether injection method. The cumulative drug release for the formulations, prepared by ether injection method, F1, F2, F3, F4, F5, and F6 were found to be 66.33%, 63.5%, 68.78%, 62.62%, 63.8%, and 61.25% respectively. The drug release kinetics studies suggest that in the formulations prepared by ether injection method,...
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Posted by admin on May 31, 2018 in |
A Reverse Phase-High Performance Liquid Chromatography method has been developed and validated for the estimation of sodium benzoate and potassium sorbate in food products. The RP-HPLC method for sodium benzoate and potassium sorbate was developed using Luna C18 column (150 mm × 4.6 mm, 5 µm) as stationary phase and acetonitrile: sodium acetate buffer pH 4.3 (20:80) as mobile phase at 1.0 ml/min flow rate and detection was carried out at 235 nm and the methods were validated in accordance with ICH guidelines. Sodium benzoate and potassium sorbate have linearity in the concentration range of 1-30 µg/ml in with correlation coefficient (r2= 0.999 & r2=0.999) respectively. Sodium benzoate and potassium sorbate eluted at 4.9 and 6.9 min, respectively. The values of LOD were 0.59 and 0.39, and LOQ were 1.8 and 1.1 for sodium benzoate and potassium sorbate respectively. Results of assay and validation study are satisfactory. So, the methods can be successfully applied for the routine analysis of sodium benzoate and potassium...
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Posted by admin on Apr 30, 2018 in |
Amlodipine besylate is a major calcium channel blocker for the treatment of hypertension. Solid dispersion Amlodipine besylate (SD-AB) was prepared by using EC, HPMC, and croscarmellose in different ratios. The development of rapidly disintegrating oral tablets of Amlodipine besylate by direct compression method. The tablets were prepared by using direct compression method and evaluated for weight variations, hardness, friability, wetting time, disintegration time, and dissolution study. Although oral disintegrating tablets are much useful in the cases of the geriatric, pediatric, and traveling patient because of no water required for the administration, therefore, the solid dispersions prepared by a solvent evaporation method using EC, HPMC and croscarmellose carrier can be successfully used for the improvement of dissolution of SD-AB and resulted in a faster onset of action as indicated by in-vivo...
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Posted by admin on Mar 31, 2018 in |
Objective: To rationalize the macroscopical and microscopical study, TLC and HPTLC fingerprinting of Ficus religiosa Linn. leaves. Methods: The crude ethanolic extract of leaves of Ficus religiosa Linn. was using the macroscopical characters powder microscopical study and Preliminary phytochemical investigation of TLC and HPTLC. Results: An attempt has been made to highlight this folk herbal medicine through present study which will assist in the identification of fresh as well as dried crude samples of leaves phytochemically, macroscopically, and microscopically. TLC and HPTLC fingerprint profiling were carried out, and the salient qualitative and quantitative parameters are reported. Conclusion: The present study will provide referential information for correct identification and help in checking adulteration in the market sample used in the preparation of various herbal medicines. The present observation will also be helpful in the macroscopical and microscopical study, TLC and HPTLC fingerprinting of Ficus religiosa Linn....
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Posted by admin on Feb 28, 2018 in |
The aim of the study was to determine the synergistic antimicrobial activity of a selected medicinal plant extract mixture composed of Calotropis gigantean (ElaWara, Family: Asclepiadaceae), Datura metel (Kalu Attana, Family: Solanaceae) and Ricinus communis (Erandu, Family: Euphorbiaceae) against selected gram positive and gram negative bacteria. In the study, antimicrobial activity was evaluated against two-gram positive organisms; Staphylococcus aureus (ATCC 95923) and group A Streptococcus spp. (ATCC 12384) and two gram-negative organisms; Escherichia coli (ATCC 95922) and Pseudomonas aeruginosa (ATCC 27853). Agar disc diffusion method was carried out to evaluate the antibacterial activity of the selected medicinal plant extract mixture, and the inhibition zone diameters were measured. Further, minimum inhibitory concentration (MIC) was evaluated using a broth microdilution method. The inhibition zones against S. aureus and group A Streptococcus spp. were observed. In contrast, inhibition zones were not demonstrated against selected gram-negative organisms. The MIC evaluated for S. aureus was 16 mg/mL by the broth dilution method. The results revealed that the selected medicinal plant extract mixture exerted synergistic...
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